Fig. 3: IDO1 drives direct binding of NF-κB and CXCL10 in EC cells.
From: IDO1 facilitates esophageal carcinoma progression by driving the direct binding of NF-κB and CXCL10
A IDO1 was co-expressed with CXCL10 based on bioinformatics analysis. B There was a significant positive correlation between IDO1 and CXCL10 expression. C, D Effect of IDO1 knockdown on mRNA and protein expression of CXCL10 in cell lines TE-1 and ECA-109. E, F IDO1 overexpression level can upregulate the expression of CXCL10 in EC cells. G According to the JASPAR CORE database, we predicted and found that CXCL10 existed binding sequence sites with transcription factor NF-κB1. H The wild type (CXCL10-WT) and mutant (CXCL10-MUT) of transcription factor binding motif were produced and transfected with NF-κB1 overexpression (NF-κB1) and negative control (NC) cells to evaluated Firefly luciferase & Renilla luciferase activity. I The effect of IDO1 overexpression on the expression of NF-κB protein in the nucleus and cytoplasm of esophageal carcinoma cells was investigate by western blotting. J We performed ChIP assay and verified that IDO1 overexpression can promote the binding of transcription factor NF-κB1 to the promoter of CXCL10. K IF assays demonstrated that the overexpression of IDO1 could drive NF-κB to enter the nucleus relative to the control. The data are represented as the mean ± SD. *p < 0.05, ***p < 0.001.
