Fig. 1: ZNF281 knockdown increases mitochondrial biogenesis in HCC cells.
From: ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma
A Western blotting for TFAM following knockdown of ZNF281 in both HLE and Huh7 cells. B Relative mtDNA content measured by qPCR in ZNF281 knockdown HLE and Huh7 cells. C Confocal laser scanning microscope analyses of mitochondrial mass in HCC cells with mitotracker green staining. Scale bars, 25 μm. D, E Left: Flow cytometry analyses of mitochondrial mass alteration with mitotracker green staining in HLE or Huh7 cells; Right: statistical analyses of fluorescence. F, G The mRNA levels of TFAM and genes encoding different ETC subunits were measured by RT-qPCR in ZNF281 knockdown HLE and Huh7 cells. H, I Immunohistochemistry of ZNF281 and ATP5B in HCC tissues in comparison with corresponding para-cancerous tissues (lower: 5×, upper: 20× objective). J ZNF281 and ATP5B IHC score correlation was analyzed. *p < 0.05, **p < 0.01, and ***p < 0.001. All experiments were performed at least three times.
