Fig. 4: ZNF281 inhibits the expression of PGC-1α-NRF1-TFAM axis in HCC cells.
From: ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma
A Upper: Scheme of TFAM promoter region containing potential ZNF281 binding motifs. Triangular arrows indicate primers. Red line represents the amplified product. Black boxes indicate GC-rich sequence. Middle: ChIP-qPCR demonstrating the enrichment of ZNF281 at TFAM promoter in HLE/Huh7 cells. Lower: Analysis of ChIP with semi-quantitative PCR and agarose gel electrophoresis. B Western blotting for NRF1 or PGC-1α following ZNF281 knockdown in HLE cells. C mRNA levels of NRF1 or PGC-1α was measured by RT-qPCR in HLE cells. D, E Upper: Scheme of NRF1 or PGC-1α promoter region containing potential ZNF281 binding motifs for ChIP analyses. Middle: ChIP-qPCR demonstrating the enrichment of ZNF281 at PGC-1α/NRF1 promoter in HLE cells. Lower: Analysis of ChIP results with semi-quantitative PCR and agarose gel electrophoresis. *p < 0.05, **p < 0.01, and ***p < 0.001. All experiments were performed at least three times.
