Fig. 5: ZNF281 regulates expression of TFAM, TFB1M and TFB2M through interaction with NRF1/PGC-1α.
From: ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma
A–C Co-immunoprecipitation (Co-IP) analyses for the interaction of ZNF281 with NRF1/PGC-1α. D Immunofluorescence showed co-localization of ZNF281 and NRF1 in HLE and Huh7 cells (bar = 10 μm). E Upper: ChIP-qPCR demonstrating the enrichment of ZNF281 in the promoter regions of TFAM/TFB1M/TFB2M harboring NRF1 binding sites. Lower: Analyses of ChIP results by semi-quantitative PCR and agarose gel electrophoresis. F The mRNA levels of TFB1M/TFB2M were measured by RT-qPCR with ZNF281 knockdown. G Upper: ChIP-qPCR demonstrating the enrichment of ZNF281 in the promoter regions of TFAM/TFB1M/TFB2M harboring NRF1 binding sites in NRF1 knockdown HLE cells. Lower: Analysis of ChIP results with semi-quantitative PCR and agarose gel electrophoresis. *p < 0.05, **p < 0.01, and ***p < 0.001. All experiments were performed at least three times.
