Fig. 6: TFAM or NRF1 knockdown attenuates ZNF281 depletion-induced mitochondrial biogenesis.
From: ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma
A, B Western blot analyses of ZNF281 and TFAM in HLE (A) and Huh7 (B) cells. C Relative mtDNA content was measured by qPCR in ZNF281 and TFAM knockdown HLE and Huh7 cells. D Relative mtDNA content was measured by qPCR in ZNF281 and NRF1 knockdown HLE cells. E Confocal laser scanning microscope analyses of mitochondrial mass in HCC cells with mitotracker green staining. Scale bars, 25 μm. F, G Left: Flow cytometry analyses of mitochondrial mass with mitotracker green staining in ZNF281 and TFAM knockdown HLE or Huh7 cells; Right: statistical analysis of mitotracker fluorescence. H mRNA levels of TFAM and genes encoding different mitochondrial ETC subunits were measured by RT-qPCR in ZNF281 and TFAM knockdown HLE and Huh7 cells. *p < 0.05, **p < 0.01, and ***p < 0.001. All experiments were performed at least three times.
