Fig. 1: The sensitivity of PCa cells to DCT was affected by DCTR-miRNAs overexpression. | Cell Death Discovery

Fig. 1: The sensitivity of PCa cells to DCT was affected by DCTR-miRNAs overexpression.

From: Secreted miR-210-3p, miR-183-5p and miR-96-5p reduce sensitivity to docetaxel in prostate cancer cells

Fig. 1

A Schematic representation of the NGS data analysis used to identify the DCTR-miRNAs in DU-145/DCTR clones. B DCTR-miRNAs relative quantification by qRT-PCR in the medium of DU-145/DCTR clones compared to parental cells medium. Effect of DCTR-miRNAs overexpression on proliferation (C–F) and colony forming ability (G, H) in 22Rv1 (C, E, G) and DU-145 (D, F, H) cells. Cell proliferation (C, D) was evaluated for each miRNA in treated (with increasing DCT doses) compared to untreated samples. Ki-67 mRNA expression level (E, F) and colony forming ability (G, H) were evaluated for each miRNA in treated (3 nM DCT) compared to untreated sample. I Kaplan–Meier curves showing progression-free interval (PFI) relative to patients stratified by sDCTR-miRNAs expression level (high/low according to 0.5 quantiles of log2(total_RPM + 1)). Data are reported as mean ± SD of at least three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ****< 0.0001 unpaired t-test (C–H). In (C, D) dP < 0.05, cP < 0.01, bP < 0.001, aP < 0.0001 (unpaired) t-test.

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