Fig. 3: sDCTR-miRNAs modify DCT response of PCa cells by regulating PPP2CB and INSIG1. | Cell Death Discovery

Fig. 3: sDCTR-miRNAs modify DCT response of PCa cells by regulating PPP2CB and INSIG1.

From: Secreted miR-210-3p, miR-183-5p and miR-96-5p reduce sensitivity to docetaxel in prostate cancer cells

Fig. 3

Cell proliferation after PPP2CB (A), INSIG1 (B), and INSIG2 (D) silencing in treated (with increasing DCT doses) compared to untreated samples. C Relative INSIG2 mRNA quantification by qRT-PCR after miR-96-5p overexpression in treated (3 nM) compared to untreated samples. E FASN, ACLY, and HMGCR mRNAs relative quantification by qRT-PCR after INSIG1 silencing or miR-183-5p or miR-210-3p overexpression in treated (3 nM DCT) compared to untreated samples. F Kaplan–Meier curves showing progression-free interval (PFI) relative to patients stratified by PPP2CB and INSIG1 expression level (high/low according to 0.5 quantile of log2(x + 1)). Log-rank test’s p-value is shown. Data are reported as mean ± SD of at least three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001 unpaired t-test (A–E).

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