Fig. 4: Secreted sDCTR-miRNAs exposure decreases the DCT sensitivity of PCa cells.
Relative sDCTR-miRNAs quantification by qRT-PCR in the exosomes secreted by 22Rv1/9.1 and /9.2 DCTR clones (A) and DU-145/3.1 and /6.7 DCTR clones (B) compared to the exosome secreted by the correspondent parental cells. Relative cell proliferation of 22Rv1 (D) or DU-145 (E) cells exposed to 22Rv1/9.1, /9.2 or DU-145/3.1, /6.7 exosomes respectively in treated (3 nM DCT) compared to untreated samples. Relative cell proliferation of 22Rv1 (G) or DU-145 (H) cells after exposure of sDCTR-miRNAs enriched exosomes in treated (3 nM DCT) compared to untreated samples. PCa cells were exposed to exosomes enriched with a single sDCTR-miRNA or a mixture of exosomes enriched with a single sDCTR-miRNA (mix miRNAs exo). Relative cell proliferation of 22Rv1 (J) or DU-145 (K) cells exposed to conditionate medium recovered after the overexpression of an equimolar mix of the three sDCTR-miRNAs (miRNAs mix medium) in treated (3 nM DCT) compared to untreated samples. C, F, I Schematic representation of the exosomes/medium exposure experiments. Data are reported as mean ± SD of at least three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001 unpaired t-test.
