Fig. 3: Knockdown of C1qA increased the Rituximab resistance of Farage/S cells, and C1qA overexpression reduced the Rituximab resistance of Farage/R cells. | Cell Death Discovery

Fig. 3: Knockdown of C1qA increased the Rituximab resistance of Farage/S cells, and C1qA overexpression reduced the Rituximab resistance of Farage/R cells.

From: METTL3-mediated m6A methylation of C1qA regulates the Rituximab resistance of diffuse large B-cell lymphoma cells

Fig. 3

Farage/S cells were transfected with shC1qA and Farage/S cells were transfected with pcDNA 3.0-C1qA. A, B The expression of C1qA in 4 groups. C The survival rates of cells treated with different concentrations of Rituximab, and the IC50 value. D The proliferation of cells treated with 20 μg/mL Rituximab was detected by the colony formation assay. E C3 chains expressed during treatment with 20 μg/mL Rituximab were detected by western blotting. F, G Apoptotic cells during treatment with 20 μg/ mL Rituximab were detected by flow cytometry and TUNEL staining. S-shCtrl, Farage/S cells transfected with shcontrol; S-shC1qA, Farage/S cells transfected with shC1qA; R-Vector, Farage/R cells transfected with an empty vector; R-C1qA, Farage/R cells transfected with the C1qA overexpression vector. ***p < 0.001, ###p < 0.001.

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