Fig. 4: Anti-H3K27me3 CUT&Tag assay of GCs isolated from the ovaries of mice treated with or without CTX for 6 h. | Cell Death Discovery

Fig. 4: Anti-H3K27me3 CUT&Tag assay of GCs isolated from the ovaries of mice treated with or without CTX for 6 h.

From: The EZH2-H3K27me3 axis modulates aberrant transcription and apoptosis in cyclophosphamide-induced ovarian granulosa cell injury

Fig. 4

A PCA analysis of the targeted H3K27me3 peaks of the GCs isolated from the ovaries of mice treated with or without CTX for 6 h. GCs isolated from 3 mice were pooled. N = 3 biological replicates. B Distribution of H3K27me3 peaks on the functional regions of genes. C Metaplot showing H3K27me3 enrichment with 2 kb upstream and downstream of the gene body in the GCs isolated from the ovaries of mice treated with or without CTX. D Heatmap showing the genes with significantly low or high H3K27me3 peaks in the GCs isolated from the ovaries of mice treated with or without CTX. E Metaplot and heatmap showing H3K27me3 enrichment of individual genes in GCs isolated from the ovaries of mice treated with or without CTX. F Distribution of significantly downregulated (top) and upregulated (bottom) H3K27me3 peaks relative to the TSS sites. G Genome browser view showing H3K27me3 enrichment near Egr4 and Klf4 in GCs isolated from the ovaries of mice treated with or without CTX. H GO analysis of significantly enriched pathways in the gene sets mapped to the downregulated H3K27me3 peaks in GCs isolated from the ovaries of mice treated with CTX.

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