Fig. 5: Protective potential of GSK-J4 against CTX-induced GCs apoptosis and transcription abnormality.

A Schematic diagram showing the grouping and the dosing schema of 3-week-old female ICR mice. GSK-J4 or DMSO was i.p. injected three times in a week (days 21, 23, and 25, as bounded by the dashed lines). PMSG were i.p. injected once at day 27. CTX or PBS was i.p. injected once 22 h after PMSG injection (day 28). The ovaries were harvested 24 h after CTX injection (day 29). B Western blot results showing the cPARP, PARP, γH2AX, and H3K27me3 levels in the PBS and CTX groups. β-tubulin, β-actin, and H3 were blotted as loading controls. N = 4 mice for each group. C Relative expression of Egr4, Klf4, Btg2, Cdkn1a, Gadd45g, and Alox5 mRNA levels by RT-qPCR in the GCs isolated from the ovaries of mice. N = 4 mice for each group. D Immunofluorescence staining of the ovaries collected from the above three groups. TUNEL was probed with Alexa Fluor 488 (green). Cell nuclei were labeled with DAPI (blue). Scale bar, 100 μm. N = 6 ovaries from different mice for each group. E Quantitative plots of TUNEL-positive follicles/total follicles. Data are the mean ± SD of at least three independent experiments. Statistical analyses were carried out using two-tailed Student’s t-test; *P < 0.05; **P < 0.01; and ***P < 0.001.