Fig. 1: Overexpression of LASS2 suppresses liver cancer tumorigenicity in vitro.

A CCK-8 assay for HCCLM3 cell line demonstrates that overexpression of LASS2 suppresses hepatoma cells proliferation. B Apoptotic DNA fragmentation in HCCLM3 cell line was detected using a dUTP TUNEL assay. Scale bar, 50 µm. Quantification results of TUNEL assay showed that overexpression of LASS2 promotes hepatoma cells apoptotic (C). Migration and invasion assay for HepG2 (D) and HCCLM3 (E) cell lines. The cell migration (F) and invasion (G) capability were significantly inhibited in LASS2-overexpressed hepatoma cells compared to negative control and Adv-GFP transfected cells. Immunofluorescence analysis for Ki67 in HepG2 (H) and HCCLM3 (I) cell lines. Scale bar, 50 µm (**P < 0.01, as indicated).