Fig. 3: LASS2 overexpression induces p53-mediated mitochondrial apoptosis in liver cancer cells.

A, B Representative immunoblots and quantitative analysis of Bax, Bcl-2, Cyto-c, caspase-3, 9, and cleaved-caspase-3, 9 in HepG2 (A) and HCCLM3 (B) cell lines. C, D Western blot (left) and quantification (right) of PUMA (a critical target for p53-mediated apoptosis), Bcl-2, Cyto-c expression in HepG2 (C) and HCCLM3 (D) cells either untreated or pretreated with p53 inhibitor PFT-α (20 μM) and following transfected with Adv-GFP or Adv-LASS2-GFP (*P < 0.05, **P < 0.01, ***P < 0.001, as indicated).