Fig. 5: LASS2 directly interacts with MDMX/MDM2. | Cell Death Discovery

Fig. 5: LASS2 directly interacts with MDMX/MDM2.

From: LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

Fig. 5

A GFP-precipitated bound proteins were visualized by staining Coomassie after SDS-PAGE. B Visualization using STRING of the net-work of interactions between LASS2 binding proteins related the p53 signaling pathway in Hepa1-6 hepatoma cells. C Western blot analysis following Co-IP experiments using HepG2 and HCCLM3 cell lines validated the interaction of LASS2 and MDM2 or MDMX. D Representative pictures of proximity ligation assay (PLA) in situ revealed endogenous interactions between LASS2 and MDM2 or LASS2 and MDMX in HepG2 (WT p53) and in (E) HCCLM3 (mutant p53) cell lines (red dots). F Quantification for number of PLA positive signals co-localized in the nucleus and cytoplasm per cell revealed that major nucleus interactions between LASS2 and MDM2 or (G) LASS2 and MDMX in HepG2 with wild-type p53 status and major cytoplasmic interactions in HCCLM3 with mutant p53 status cell lines. H Protein–protein docking between LASS2 and MDM2 or (I) MDMX was visualized by the web server ClusPro 2.0. The surface of LASS2 is colored in green, and MDM2 or MDMX is colored in blue. Yellow dashed line represents hydrogen bonding interactions. J Schematic diagram of the binding sites of LASS2 to MDM2 or MDMX (*P < 0.05, as indicated).

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