Fig. 6: LASS2 inhibits MDM2/MDMX expression and enhances p53 stability in hepatoma cells. | Cell Death Discovery

Fig. 6: LASS2 inhibits MDM2/MDMX expression and enhances p53 stability in hepatoma cells.

From: LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX

Fig. 6

A, B Disease Free Survival (DFS) analysis of (A) MDM2 and (B) MDMX expression in patients with liver cancer using the Kaplan–Meier Plotter database. C, D A analysis of the relationship between LASS2 and (C) MDM2 or (D) MDMX demonstrated that LASS2 transcript levels were negatively correlated with MDM2 or MDMX in the TNMplot database (https://www.tnmplot.com/). E, F LASS2 overexpression inhibits the mRNA levels of (E) MDM2 or (F) MDMX in HepG2 and HCCLM3 cells. G, H Representative western blotting images (left) and quantification (right) of MDM2 and MDMX in (G) HepG2 and (H) HCCLM3 cell lines. I Dual luciferase assays show that LASS2 overexpression increase the transcriptional activity of p53. J, K Representative western blotting images (J) and quantification (K) of p53 protein in the nucleus and cytoplasm of HepG2 with WT p53 or HCCLM3 with p53 mutant cells (*P < 0.05, **P < 0.01, ***P < 0.001, as indicated).

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