Fig. 2: In vitro drug sensitivity is reproducible independently of operator and screening platform.

KARPAS1718 cells were screened against a drug library consisting of 88 (NCMM and FIMM libraries) or 97 (DK-OPENSCREEN library) single agents and 95 (NCMM and FIMM libraries) or 102 (DK-OPENSCREEN library) drug combinations at five different concentrations. Cell viability was assessed with the CellTiter-Glo luminescent cell viability assay after 72 h. The drug sensitivity score (DSS) was calculated for each treatment based on the area under the dose-response curve. A high score indicates high sensitivity to the treatment. a Overview of the performed experiments. Experiment number (1–4), operator (A or B), plate source (I, NCMM; II, FIMM; III, DK-OPENSCREEN) and Z-prime on the four assay plates (A-D) are indicated. b Simple linear regression was performed for the responses to the combinations on plate C (Z-prime > 0.5) of the drug library, for the indicated experiments (1, 2, 4). Operator and plate source for each experiment are highlighted. c The DSS for single agents on Plate A are shown for experiments with Z-prime > 0.5 (1 and 2, left panel) and < 0.5 (3 and 4, right panel). d The cell viability relative to DMSO (0.1%) treated control cells are shown for KARPAS1718 cells treated with the indicated drugs. These agents were located on Plate A, which had a low Z-prime in experiment 3 (−2.53; coral curve) and experiment 4 (0.41; black curve). In experiments 1 and 2, the Z-prime for Plate A was > 0.5. e Simple linear regression was performed for the responses to the single agents of the drug library, for the indicated experiments (1, 2, 3). Responses to single agents on Plate A are highlighted in coral (left panel), while responses to single agents on plates B-D are shown in black. In the right panel, the responses to single agents on Plate A of experiment 3 are replaced by responses to the same agents from experiment 2 (blue data points).