Fig. 1: Diabetic wounds display a prolonged healing process and senescent phenotype. | Cell Death Discovery

Fig. 1: Diabetic wounds display a prolonged healing process and senescent phenotype.

From: PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway

Fig. 1

A Gross examination of the wound area in diabetic mice and the control group at the indicated time points. B The skin wound area of diabetic mice and the control group during the wound healing process (n = 4, expressed as the percentage of the initial wound area). C Wound healing time of two groups (n = 4). D HE staining (scale bar, 1 mm) and E Masson’s trichrome staining (scale bar, 100μm) of day-10 skin wounds of two groups. F, G PDK4, senescence markers and SASP-related markers were detected by RT-qPCR and Western blot in skin wound tissues of diabetic mice and the control group (n = 4; *, **, ***, compared to Day 0 in the control group; #, ##, ###, compared to Day 0 in the DM group). H Representative images of SA-β-Gal+ (blue) cells, depicted by black arrows from skin wound tissues of a diabetic patient (59 years old, a 3-mm skin biopsy was taken from the 10-day wound edge on the lower extremity) and a non-diabetic patient (51 years old, a 3-mm skin biopsy was taken from the 10-day wound edge on the lower extremity caused by a traffic accident) (scale bar, 50 μm). The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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