Fig. 3: PDK4 overexpression accelerates diabetic wound healing and improves the senescence phenotype. | Cell Death Discovery

Fig. 3: PDK4 overexpression accelerates diabetic wound healing and improves the senescence phenotype.

From: PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway

Fig. 3

A Western blot analysis of PDK4 to confirm efficient transduction. B Gross examination of the wound area at the indicated time points. C The skin wound area during the wound healing process (n = 4, expressed as the percentage of the initial wound area) D Wound healing time of two groups (n = 4). E HE staining (scale bar, 1 mm) and F Masson’s trichrome staining (scale bar, 100 μm) of day-10 skin wounds. G Angiogenesis evaluated by CD31 immunohistochemical analysis (n = 4, scale bar, 50 μm). H Proliferation evaluated by Ki67 immunohistochemical analysis (n = 4, scale bar, 50 μm). I Senescence markers and SASP-related protein detected by western blot in mice skin wound tissues (n = 4). Data are shown as mean ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant.

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