Fig. 5: PDK4 suppresses high-glucose-induced senescence in HDFs through YAP nuclear translocation. | Cell Death Discovery

Fig. 5: PDK4 suppresses high-glucose-induced senescence in HDFs through YAP nuclear translocation.

From: PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway

Fig. 5

A Western blot analysis of protein level of p-YAP, YAP, and CTGF in HDFs (n = 3). B Level of YAP detected by western blot in cytoplasmic and nuclear extraction. Histone H3 was used as a nuclear internal reference, and β-tubulin was used as a plasma reference (n = 3). C, D RT-qPCR and western blot analysis of YAP to verify the efficiency of knockdown and overexpression (n = 3). E Senescence markers and SASP-related protein detected by western blot in HDFs (n = 3). F, G SA-β-GAL staining images of different groups of HDFs (n = 3). H Cell proliferation assessed by cell counting kit-8 assay (n = 3, **P < 0.01, ***P < 0.001, compared to the HG+oe-PDK4 group; ##p < 0.01, ###p < 0.001, compared to the HG+oe-NC group;). I, J Transwell assay images and quantitative analysis of the migration of HDFs (n = 3, scale bar, 200μm). Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; compared to the ED-EVs group, ###p < 0.001.

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