Fig. 7: PDK4-induced ROS reduction mediates YAP nuclear translocation and JNK dephosphorylation. | Cell Death Discovery

Fig. 7: PDK4-induced ROS reduction mediates YAP nuclear translocation and JNK dephosphorylation.

From: PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway

Fig. 7

A, B Representative fluorescence images of reactive oxygen species (ROS) staining in HDFs (scale bar, 200 μm). C p-YAP, YAP, p-JNK, JNK, senescence markers and SASP-related protein detected by western blot in HDFs (n = 3). D Level of YAP detected by Western Blot in cytoplasmic and nuclear extraction. Histone H3 was used as a nuclear internal reference, and β-tubulin was used as a plasma reference (n = 3). E SA-β-GAL staining images of different groups of HDFs (n = 3). F Cell proliferation assessed by cell counting kit-8 assay (n = 3, **P < 0.01, ***P < 0.001, compared to the HG + LV-PDK4 group; ##p < 0.01, ###p < 0.001, compared to the HG group;). G Transwell assay images and quantitative analysis of the migration of HDFs (n = 3, scale bar, 200 μm). Data are shown as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant.

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