Fig. 2: Overexpression of ESRP1 inhibits migration and invasion of gastric cancer in vitro and in vivo.
From: ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer
A Transwell migration and invasion assay of control (oeVector) and ESRP1 overexpression (oeESRP1) gastric cancer cells. The left was SGC7901 gastric cancer cell and right was BGC823 cell. B Statistical histogram of the number of penetrated cells transwell migration and invasion assay. Left was SGC7901 and right was BGC823. C Western blot detecting the expression of Ecadherin, Ncadherin, ESRP1, and GAPDH protein in control (oeVector) and ESRP1 over expression (oeESRP1) gastric cancer cells. Left was SGC7901 cell and right was BGC823 cell. D In vivo analysis of ESRP1 overexpression influencing gastric cancer metastasis. 1 × 106 oeVector or oeESRP1 SGC7901 cells were injected through tail vein (n = 5) and 1.5 months later, mice were sacrificed and lungs were dissected and fixed with Bouin’s Solution. The representative images were showed. Red arrows indicate lung metastases. E Number of metastases on the lung surface were counted and compared between oeVector and oeESRP1 groups. F Representative images of H&E staining of lungs in mice injected oeVector or oeESRP1 SGC7901 cells. G Representative images of IHC staining of Ecadherin in the lung metastases. Left was oeVector and right was oeESRP1 group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
