Fig. 7: CLSTN1 affect the interaction of Ecadherin and β-catenin structure.
From: ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer
A Co-immunoprecipitation of CLSTN1 and β-catenin protein in SGC7901 gastric cancer cells. IB: immune blotting. B co-immunoprecipitation of β-catenin and Ecadherin, CLSTN1-F, CLSTN1-S in SGC7901 oeVector, oeCLSTN1-F, and oeCLSTN1-S cells. IP: immunoprecipitation. C co-immunoprecipitation of β-catenin and Ecadherin in SGC7901 oeESRP1 gastric cancer cells. D Immunofluorescence co-localization of β-catenin and Ecadherin in SGC7901 oeVector or oeESRP1 cells. Representative images were shown. E CHX assay of SGC7901 gastric cancer cells oeVector or oeESRP1. F Ni-NTA pulldown of HEK293T cells transfected with ESRP1 and/or ubiquitin plasmids. ESRP1 (Flag), Flag-tagged ESRP1 plasmid; Ub (His), His-tagged ubiquitin plasmid. G Ni-NTA pulldown of HEK293T cells transfected with CLSTN1-F, CLSTN1-S, and/or ubiquitin plasmids. CLSTN1-F (Flag), Flag-tagged CLSTN1-F plasmid; CLSTN1-S (Flag), Flag-tagged CLSTN1-S plasmid.
