Fig. 4: Inhibition of CB2 alleviated osteoclast activation through NOX1-mediated oxidative stress signaling. | Cell Death Discovery

Fig. 4: Inhibition of CB2 alleviated osteoclast activation through NOX1-mediated oxidative stress signaling.

From: CB2 regulates oxidative stress and osteoclastogenesis through NOX1-dependent signaling pathway in titanium particle-induced osteolysis

Fig. 4

A RT-PCR analysis of the mRNA expression levels of NOX1, Nrf2, SOD2, HO-1, and NOX2. B RT-PCR was used to detect the inhibitory effect of ML171 intervention on NOX1 expression in RAW264.7. C RT-PCR analysis of the mRNA expression levels of CTSK, NFATc1, Atp6v0d2, and MMP9. D, G Representative TRAcP staining and quantitative analysis were used to detect the effects of Cnr2-689 and ML171 on osteoclast differentiation. E, H Immunofluorescence staining revealed the effects of Cnr2-689 and ML171 on the expression of NFATc1. F, I SEM images of the bone resorption area. J, L Immunofluorescence staining and quantitative analysis of MitoTracker Red. K, M Immunofluorescence staining and quantitative analysis of ROS. The data were represented as mean ± SD of three independent experiments. Statistical significance was determined by a one-way ANOVA with Tukey’s multiple-comparison test. *P < 0.05, **P < 0.01.

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