Fig. 6: Inhibition of CB2 inhibited the number of osteoclasts and ROS levels in osteolytic mice.

A H&E staining of skulls, Scale bar = 100 µm. B, E TRAcP staining of the skull and quantitative analysis of osteoclasts in each group of mice. Scale bar = 100 µm. C, F ROS staining and quantitative analysis of immunofluorescence staining in each group of mice, Scale bar = 100 µm. D, G, H Immunohistochemical staining and quantitative analysis of NOX1 and NFATc1 in decalcified bone sections. n = 7 per group. The data were represented as mean ± SD. Statistical significance was determined by a one-way ANOVA with Tukey’s multiple-comparison test. *P < 0.05, **P < 0.01.