Fig. 6: Preparation and characterization of PRT@PPMP.

A Schematic illustrating the design of the PRT@PPMP. B Electron microscopic images of PRT@PPMP. C Size distribution. D The surface charge of PPM, PRT@PPM, and PRT@PPMP determined using the zeta potential and dynamic light scattering. E Fluorescence microscopy revealing the cellular uptake of PRT@PPMP by CMECs and CMs. F Immunofluorescence images showing intracellular localization of PRT@PPM and PRT@PPMP within CMs at different time points. Green-labeled PRT@PPM and PRT@PPMP with DAPI-stained nuclei (blue). G Cellular uptake of PRT@PPM and PRT@PPMP by CMs, observed through flow cytometry analysis. Data are presented as mean ± SD (n = 6). Scale bars: 25 μm.