Fig. 8: Targeted release of PRT in the myocardium prevents cardiac dysfunction. | Cell Death Discovery

Fig. 8: Targeted release of PRT in the myocardium prevents cardiac dysfunction.

From: Upregulation of Hsp27 via further inhibition of histone H2A ubiquitination confers protection against myocardial ischemia/reperfusion injury by promoting glycolysis and enhancing mitochondrial function

Fig. 8

A Scheme of the time axis representing the design of the animal study. B Representative echocardiographic images from each group. C, D Echocardiographic parameters, such as LVEF and LVFS, were calculated in sham and reperfusion rats treated with or without different concentrations of PRT@PPMP. E Immunofluorescence study depicting the delivery efficiency of Cy5.5-stained PRT@PPM or PRT@PPMP to the heart 6 h after individualized treatment. F Cy5.5-stained PRT@PPMP accumulated in the heart and other organs. G Representative images of the heart sections labeled with Hsp27 fluorescent probe. DAPI (blue, nuclei); Hsp27 positive (red). H Representative morphological analysis using HE staining. I–J TTC staining revealing the area of myocardial infarction in rats. K ROS levels in the cardiac tissue. L Electron microscopic analysis of myocardial cells revealing changes in mitochondrial morphology. M MDA levels in the cardiac tissue. N Relative mRNA levels of ptgs2 measured in cardiac tissue using qRT-PCR. Data are presented as mean ± SD (n = 6). #P < 0.05 vs. Sham group; *P < 0.05 vs. Reperfusion group. Scale bars: (G, H) 50 μm; (L) 2.0 μm.

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