Fig. 2: Hypoxia stimulates pyroptosis in macrophages. | Cell Death Discovery

Fig. 2: Hypoxia stimulates pyroptosis in macrophages.

From: CCL11 released by GSDMD-mediated macrophage pyroptosis regulates angiogenesis after hindlimb ischemia

Fig. 2

A Western blots of GSDMD-FL, GSDMD-N, IL-1β, and IL-18 protein expression levels in normoxia and hypoxia HUVECs. B The relative mRNA levels of GSDMD, NLRP3, IL-1α, IL-1β, IL-18, TNF-α, and HMGB1 of hypoxia THP-1 cells compared to normoxia THP-1 cells. C Western blots of GSDMD-FL and GSDMD-N protein expression levels in normoxia and hypoxia THP-1 cells. D The level of LDH in cell culture supernatants detected by LDH release test. E The level of IL-1β in cell culture supernatants detected by ELISA. n = 3 for each group; ns represents p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.0001.

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