Fig. 7: CCL11 facilitates endothelial cell proliferation, migration, and in vitro angiogenesis.

A, B Representative images and quantification of chemotaxis of HUVECs toward CCL11 were assessed with transwell assay. There was a dose-dependent increase of chemotaxis toward CCL11. C Relative cell proliferation rate of HUVECs stimulated with conditioned medium collected from hypoxia THP-1 cells, CCL11, and CCL11 antibody (anti-CCL11). D, E Representative images and quantification of wound closure for 24 h of HUVECs stimulated with the conditioned medium, CCL11, and anti-CCL11. F, G Tube formation assay and quantification of tube length in HUVECs stimulated with the conditioned medium, CCL11, and anti-CCL11. The number of cells in transwell assay was counted by Image Pro Plus; the values of relative wound closure and tube length were measured by ImageJ. Scale bar = 50 μm. n = 3 for each group; ns represents p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.0001.