Fig. 2: Most significantly altered proteins identified by proteomic profiling following 1,4 dihydroxy quininib treatment in OMM2.5 cells and immunoblot validation. | Cell Death Discovery

Fig. 2: Most significantly altered proteins identified by proteomic profiling following 1,4 dihydroxy quininib treatment in OMM2.5 cells and immunoblot validation.

From: 1,4-dihydroxy quininib activates ferroptosis pathways in metastatic uveal melanoma and reveals a novel prognostic biomarker signature

Fig. 2

A Table showing the 10 most up- and down-regulated proteins in OMM2.5 cells after 4 h of treatment with 20 µM Q7. B Table showing the 10 most up- and down-regulated proteins in OMM2.5 cells after 8 h of treatment with 20 µM Q7. C Table showing the 10 most up- downregulated proteins in OMM2.5 cells after 24 h of treatment with 20 µM Q7. Protein names coloured in red and blue in tables A, B and C are Q7-upregulated and downregulated proteins, respectively, known to be associated to the ferroptosis pathway. D Immunoblot analysis of HO-1 and IREB2 in protein extracts from OMM2.5 cells treated with 0.5% DMSO or 20 µM Q7 for 4, 8 or 24 h. E Densitometric quantification of HO-1 and IREB2 normalized to beta-actin, as determined by n = 4 independent western blot experiments as in D (*, p < 0.05). F Immunoblot analysis of GDF-15 in protein extracts from OMM2.5 cells treated with 0.5% DMSO or 20 µM Q7 OMM2.5 cells for 4, 8 or 24 h. G Densitometric quantification of GDF15 normalized to beta-actin, as determined by at least three independent western blot experiments as in F (*, p < 0.05). The histograms report mean ± SEM. Q7 = 1,4-dihydroxy quininib; h = hours.

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