Fig. 5: NMDAR and PP2A-AMPK-HMGB1 pathway mediate VEC ferroptosis in vivo.
From: NMDARs activation regulates endothelial ferroptosis via the PP2A-AMPK-HMGB1 axis
A Experimental design summarizing the procedure. B Ultrastructure of the mitochondria of the vascular tissue of mice treated with GLU (1 g/kg) or NMDA (75 mg/kg) for 10 days, visualized by TEM. Black arrowheads: shrunken mitochondria. Scale bars, 5 μm. The relative levels of (C) iron, (D) GSH, (E) MDA, (F) LPO, and (G) GPX4 content in the vascular tissue of the treated mice. H Western blot image and (I) quantification of PTGS2, GPX4, and SLC7A11 in NMDA and/or LB100-, AICAR-, MK-801-, and GLY-treated mice. ***P < 0.001 vs control; #P < 0.05, ##P < 0.01 and ###P < 0.001 vs NMDA, &P < 0.05, &&P < 0.01 and &&&P < 0.001 vs GLU. J Western blot image and (K) quantification of PTGS2, GPX4, and SLC7A11 in GLU and/or LB100-, AICAR-, MK-801-, and GLY-treated mice. *P < 0.05, **P < 0.01 and ***P < 0.001 vs control; #P < 0.05, ##P < 0.01 and ###P < 0.001 vs NMDA/GLU in PTGS2 expression; &P < 0.05, &&P < 0.01 and &&&P < 0.001 vs NMDA/GLU in GPX4 expression.
