Fig. 1: Successful construction of MYOCS341P transgenic mice. | Cell Death Discovery

Fig. 1: Successful construction of MYOCS341P transgenic mice.

From: Serine to proline mutation at position 341 of MYOC impairs trabecular meshwork function by causing autophagy deregulation

Fig. 1

A Alignment of MYOC protein sequences in different species. B Alignment of MYOC mutation site in different species. C MYOC gene and protein structures. The schematic diagram shows the location of the missense mutation in MYOC. D Sanger sequencing of genomic DNA from MYOCS341P transgenic mice (n = 3). E Agarose gel electrophoresis verification of transgene integration in MYOCS341P transgenic mice (n = 17). F Western blot analysis of MYOC, GRP78, GRP94, P62, and LC3B expression in anterior chamber angle tissue from MYOCS341P transgenic mice (n = 3) and wild-type (WT) littermates (n = 3). G Immunostaining of MYOC, KDEL, and P62 in anterior chamber angle tissue in MYOCS341P transgenic mice (n = 3) and WT littermates (n = 3).

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