Fig. 5: Decreased cell viability and increased cell death in MYOC^S341P overexpressing human trabecular meshwork (HTM) cells.

A Immunofluorescence staining of LAMA 4 (red) and TIMP3 (green) in HTM cells. B Western blot detection of MYOC and GAPDH, with or without dexamethasone treatment (left); MYOC band intensity was normalized to that of the untreated group using ImageJ software (right). **p < 0.01; ***p < 0.001. C Western blot detection of MYOC and GAPDH in HTM cells overexpressing MYOC^WT or MYOC^S341P (left); MYOC band intensity was normalized to the empty vector group using ImageJ software (right). ***p < 0.001. Empty, empty vector lentiviruses; MYOC^WT, lentiviruses expressing the wild-type (WT) myocilin gene; MYOC^S341P, lentiviruses expressing the S341P mutant myocilin gene. D, E Cell viability was detected by CCK8 assay in two strains of HTM cells overexpressing MYOC^WT or MYOC^S341P. **p < 0.01. MYOC^WT, lentiviruses expressing the WT myocilin gene; MYOC^S341P, lentiviruses expressing the S341P mutant myocilin gene. F Flow cytometry analysis of propidium iodide (PI)-positive cells in HTM1 after MYOC^WT or MYOC^S341P overexpression (left). Statistical analysis of the proportion of positive cells (right). ***p < 0.001. MYOC^WT, lentiviruses expressing the WT myocilin gene. MYOC^S341P, lentiviruses expressing the S341P mutant myocilin gene. G Flow cytometry analysis of PI-positive cells in HTM2 after MYOC^WT or MYOC^S341P overexpression (left). Statistical analysis of the proportion of positive cells (right). ***p < 0.001. MYOC^WT, lentiviruses expressing the WT myocilin gene. MYOC^S341P, lentiviruses expressing the S341P mutant myocilin gene.