Fig. 6: Induced ER stress in HTM cells overexpressing MYOC^S341P.

A Western blot analysis of MYOC^WT or MYOC^S341P after co-immunoprecipitation. B Analysis of proteins differentially interacting with MYOC^WT and MYOC^S341P. C Analysis of the biological functions of proteins differentially interacting with MYOC^WT and MYOC^S341P. D Western blot detection of GRP78, GRP94, and GAPDH in two strains of HTM cells overexpressing MYOC^WT or MYOC^S341P. MYOC^WT, lentiviruses expressing the wild-type (WT) myocilin gene. MYOC^S341P, lentiviruses expressing the S341P mutant myocilin gene. E Western blot detection of MYOC in two strains of HTM cells overexpressing MYOC^WT or MYOC^S341P (upper). Coomassie staining analysis of total proteins in two strains of HTM cells overexpressing MYOC^WT or MYOC^S341P (lower). MYOC^WT, lentiviruses expressing the WT myocilin gene. MYOC^S341P, lentiviruses expressing the S341P mutant myocilin gene. F, G Immunofluorescence staining of MYOC (red) and KDEL (green) in two strains of HTM cells overexpressing MYOC^WT or MYOC^S341P.