Fig. 7: Impaired autophagy in HTM cells overexpressing MYOC^S341P.

A RNA-seq analysis of genes differentially expressed between MYOC^WT and MYOC^S341P. B KEGG analysis of proteins differentially expressed between MYOC^WT and MYOC^S341P. C Western blot detection of LC3B, P62, and GAPDH in two strains of HTM cells overexpressing MYOC^WT or MYOC^S341P. WT, lentiviruses expressing the wild-type (WT) myocilin gene. S341P, lentiviruses expressing the S341P mutant myocilin gene. D–F HTM cells were transfected with lentiviruses expressing MYOC^WT or MYOC^S341P for 48 h, then transduced with Ad5-mCherry-GFP-LC3 for 24 h. GFP dots, mCherry dots, yellow dots, and free mCherry dots were counted. **p < 0.01; ***p < 0.001, ****p < 0.0001. WT, lentiviruses expressing the WT myocilin gene. S341P, lentiviruses expressing the S341P mutant myocilin gene. G Western blot detection of LC3B, P62, and GAPDH in HTM cells overexpressing MYOC^WT or MYOC^S341P, with or without rapamycin treatment. WT, lentiviruses expressing the WT myocilin gene; S341P, lentiviruses expressing the S341P mutant myocilin gene. H Immunofluorescence staining of MYOC (red) and KDEL (green) in two HTM cells overexpressing MYOC^WT or MYOC^S341P without Rap treatment (upper) or with Rap treatment (lower).