Fig. 2: Analysis of apoptotic process in RTD iPSCs.
From: Caspase-dependent apoptosis in Riboflavin Transporter Deficiency iPSCs and derived motor neurons
Act casp-3 IF (Scale bar in A, 25 µM; in B, 5 µM). C TUNEL assay (Scale bar, 50 µm). Nuclei are stained with HOECHST (blue) while TUNEL and Act casp-3 positive cells are marked in green. D Quantification of Act casp-3 fluorescent signal intensity. A minimum of n = 3 and 9 images were quantized. E WB analysis of Act casp-3 and GAPDH (as loading control). Bar graph represents the mean ± SEM of three experiments (n = 3). F Quantitative analysis of TUNEL apoptotic assay (performed in triplicate). A minimum of 12 images and 4000 cells were counted. Data were analyzed by Student’s t tests and Kruskal-Wallis test ± SEM. ****p ≤ 0.0001; ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05.
