Fig. 3: Effects of ARK-5-KO on apoptosis and cell cycle in myeloma cells. | Cell Death Discovery

Fig. 3: Effects of ARK-5-KO on apoptosis and cell cycle in myeloma cells.

From: ARK5 enhances cell survival associated with mitochondrial morphological dynamics from fusion to fission in human multiple myeloma cells

Fig. 3

A Apoptosis assay via annexin V-based staining method. The proportion of apoptotic cells was higher in ARK5-KO cells than in ARK5-WT cells. The right bar graphs represented the proportion of apoptotic (Annexin V+/PI+) cells. The data were presented as the mean ± SEM (n = 3). Asterisks (*) show statistically significant differences (*P < 0.05). B Cell cycle was analyzed via LSRFortessa X-20 Flow Cytometer and FlowJo software. S-phase ratio was elevated in ARK5-KO cells compared to their parental cells. C Western blot analysis reflecting protein expression of ARK5, phosphorylation levels of AKT, cyclin-dependent kinase (CDK)2, CDK4, p27, caspase 9, and caspase 3 in parental (KMS-11 and Sachi), KMS-11-ARK5-KO and Sachi-ARK5-KO cells. D Effect of exogenous ARK5 expression in ARK5/ARK5-KO cells (rescue) on the protein levels of ARK5, phosphorylation levels of AKT, CDK2, CDK4, p27, caspase 9, and caspase3 compared with ARK5-KO cells in KMS-11 and Sachi.

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