Fig. 5: Effects of ARK-5-KO on the expression of GTPase associated with mitochondrial morphology in myeloma cells. | Cell Death Discovery

Fig. 5: Effects of ARK-5-KO on the expression of GTPase associated with mitochondrial morphology in myeloma cells.

From: ARK5 enhances cell survival associated with mitochondrial morphological dynamics from fusion to fission in human multiple myeloma cells

Fig. 5

A Mitofusin-1 (MFN1) (green) and dynamin-related protein 1 (DRP1) (red) were co-stained using Hoechst (blue) in human myeloma cells. MFN1 was increased in ARK5-KO cells compared to that in ARK5-WT cells and ARK5/ARK5-KO cells. DRP1 decreased in ARK5-KO cells compared to that in ARK5-WT cells and ARK5/ARK5-KO cells. B, C Western blot analysis of MFN1, MFN2, optic atrophy 1 (OPA1), phosphorylated DRP1 at serine 616, and DRP1 in parental and ARK5‐KO clone cells and ARK5/ ARK5-KO cells. D, E Reactive oxygen species (ROS) production was measured via dichloro-dihydro-fluorescein diacetate assay and mitochondria ROS assay in parental and ARK5‐KO clone cells. F Adenosine triphosphate (ATP) production was evaluated using ATPlite Luminescence assay. The bar graphs present ATP levels relative to the parent ARK5-WT cells. G Relative lactate levels to the parent ARK5-WT cells. The data were shown as the mean ± SEM (n = 3). Asterisks (*) indicate statistically significant differences (*P < 0.05).

Back to article page