Fig. 3: Hu-α-syn PFF clearance in young and senescent glia in vitro. | Cell Death Discovery

Fig. 3: Hu-α-syn PFF clearance in young and senescent glia in vitro.

From: Glial senescence enhances α-synuclein pathology owing to its insufficient clearance caused by autophagy dysfunction

Fig. 3

A Glia was treated with hu-α-syn PFF for 1 h, washed out with PBS, replaced with fresh medium, and harvested at 0, 1, 2, 6, and 24 h after medium replacement. B Representative western blotting for remaining hu-α-syn and actin of young and senescent glia at 0, 1, 2, 6, 24 h following treatment with hu-α-syn PFF. C Quantification of remaining hu-α-syn of young and senescent glia at different time points (n = 4). D Representative images of hu-α-syn deposits in young and senescent glia at the different time points (scale bar: 10 μm). E Quantification and statistical results of hu-α-syn deposits in astrocytes and microglia of young and senescent glia (n = 6). Values are presented as means ± SEMs and statistical significance was determined by two-way ANOVA with the Bonferroni test. *p < 0.05, **p < 0.01.

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