Fig. 1: Identification of clonal phage-induced THP-1 cell differentiation by phage display. | Cell Death Discovery

Fig. 1: Identification of clonal phage-induced THP-1 cell differentiation by phage display.

From: Generation of a new therapeutic d-peptide that induces the differentiation of acute myeloid leukemia cells through A TLR-2 signaling pathway

Fig. 1

A Flowchart of phage display. B The percentage of adherent THP-1 cells induced by phage libraries or PMA. C, D THP-1 cells were treated with either PMA, phages obtained from Round 3, or an empty vector for 48 h. Flow cytometry was utilized to assess apoptosis via Annexin V/propidium iodide (PI) staining (C) and proliferation via Ki-67 staining (D). E THP-1 cells were stimulated with phage library or PMA for 48 h, and empty-treated cells were used as a negative control. The suspended cells were gently removed through a PBS wash. The morphological features were then visualized by phase contrast microscopy. F The enrichment of clonal phages during different rounds of phage display. G The percentage of adherent THP-1 cells induced by different clonal phages enriched in Round 3. The results are shown as the mean and SEM. ***P < 0.001 (Kruskal–Wallis test). Data are from 6 independent experiments.

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