Fig. 5: d-pep-P6 induced differentiation of THP-1 cells through TLR-2 signaling pathway. | Cell Death Discovery

Fig. 5: d-pep-P6 induced differentiation of THP-1 cells through TLR-2 signaling pathway.

From: Generation of a new therapeutic d-peptide that induces the differentiation of acute myeloid leukemia cells through A TLR-2 signaling pathway

Fig. 5

A Expression of the candidate proteins was measured by real-time PCR after induction by d-pep-P6, compared to the untreated group. B Western blot analysis of co-IP d-pep-P6 with TLR-2 using anti-TLR-2 mAb. C Knock out the efficiency of TLR-2 by siRNA was measured by real-time PCR. A scrambled SiRNA (ScRNA) was used as a control. D d-pep-P6 were incubated with THP-1 cells that knock out TLR-2 by siRNA or ScRNA, and pep-NC treated cells were used as a negative control. Cell surface markers CD11b and CD14 were detected by FACS after 48 h incubation. Each bar represents the mean and SEM from three independent experiments in triplicate. ***P < 0.001 (Kruskal–Wallis test). MFI median fluorescent intensity. Data are from 3 independent experiments (A–C) or 6 independent experiments (D).

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