Fig. 3: Deposition of p-α-syn after stimulation with α-syn PFF induced decreased viability and apoptosis in RSC96 cells. | Cell Death Discovery

Fig. 3: Deposition of p-α-syn after stimulation with α-syn PFF induced decreased viability and apoptosis in RSC96 cells.

From: Phosphorylated α-synuclein deposited in Schwann cells interacting with TLR2 mediates cell damage and induces Parkinson’s disease autonomic dysfunction

Fig. 3

A Representative TEM images of pre-sonicated α-syn PFF and postsonicated PFF, scale bar=200 nm. B Cell viability at 24 h, 36 h, and 48 h after α-syn PFF stimulation was detected by CCK8 assay. n = 4. C Representative western blot bands of oligomer α-syn, monomer α-syn and p-α-syn. D–F The quantitative analysis of monomer α-syn, oligomer α-syn, and p-α-syn. n = 4. G Representative immunofluorescence images of p-α-syn in RSC96 cells stimulated with α-syn PFF for 24 h, scale bar=10 μm, enlarged scale bar=5 μm. H, I Representative images of S100β+ RSC96 cells stimulated with α-syn PFF for 24 h, and the quantitative analysis of cell count, scale bar=20 μm. n = 4. J, K Representative images of TUNEL staining, and the quantitative analysis of apoptosis rate in PBS or α-syn PFF group, scale bar=50 μm, enlarged scale bar=10 μm. n = 4. Data were presented as mean ± SEM and analyzed using Student’s t-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. Cell experiments were repeated three times independently. α-syn PFF α-synuclein preformed fibril, TEM transmission electron microscopy.

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