Fig. 8: TGF-β induces ROR1-dependent BMI1 activation.

A Immunoblot analysis of ROR1 protein using lysates from parental and si-ROR1 treated MB-231 cells that were stimulated with TGF-β for the indicated time with the quantification results below. B BMI1 mRNA levels in MB-231 cells treated with TGF-β at 50 ng/mL and si-control and si-ROR1 at an indicated time interval were examined by quantitative PCR (qPCR). The figure shows the mean expression of BMI1 compared to time “0”. Experiments were performed in triplicate unless indicated otherwise and normalized with GAPDH. The error bars indicate the ±SEM. C Immunoblot analyses of BMI1 protein using lysates from parental and si-ROR1 treated MB-231 cells that were stimulated with TGF-β for the indicated times and quantification results below. D Immunoblot analyses of BMI1 protein using lysates from parental and si-control and si-lncRNA DLEU2-treated MB-231 cells that were stimulated with TGF-β. E The bar graph indicates the average number of spheroids formed by parental or si-ROR1 cells treated with or without TGF-β in triplicate ±SEM. P values were calculated using a two-tailed paired Student’s t-test. **P < 0.01) F The bar graph indicates the average number of invaded cells from parental or si-ROR1 cells treated with or without TGF-beta in triplicate ±SEM. P values were calculated using a two-tailed paired Student’s t-test. **P < 0.01).