Fig. 2: Sub-FS stimuli may aggravate neuronal damage and apoptosis caused by subsequent FS stimuli.

A, B Number of FJB-positive signals and representative images of FJB staining in the EC at 3 days after FS stimuli (n = 4/group, scale bar = 50 μm). C–E Gray bands and normalized gray values of cleaved caspase-3 and caspase-3 relative to GAPDH (n = 4/group) at 3 days after FS stimuli. Mean ± SEMs were presented. *P < 0.05, **P < 0.01 vs control group (One-way ANOVA).