Fig. 4: Reduced TSP-1 expression decreases susceptibility and neuronal injury induced by sub-FS stimuli and alleviates neuronal damage induced by subsequent FS stimuli. | Cell Death Discovery

Fig. 4: Reduced TSP-1 expression decreases susceptibility and neuronal injury induced by sub-FS stimuli and alleviates neuronal damage induced by subsequent FS stimuli.

From: Increased thrombospondin-1 levels contribute to epileptic susceptibility in neonatal hyperthermia without seizures via altered synaptogenesis

Fig. 4

A Experimental design. B Threshold dosages of PTZ at P14 (n = 6/group). C Threshold body temperature for induction of febrile seizures at P9 (n = 8/group). D, E Analysis and representative images of FJB staining at 3 days after 41°C stimulus (scale bar = 50 μm). F–H Gray bands and normalized gray values of cleaved caspase-3 and caspase-3 relative to GAPDH at 3 days after 41°C stimulus. I, J Representative images (scale bar = 50 μm) and number of positive signals of FJB staining at 3 days after FS stimuli. K–M Gray bands and quantitative analysis of cleaved caspase-3 and caspase-3 at 3 days after FS stimuli. D–M n = 4/group. Mean ± SEMs were presented. *P < 0.05, **P < 0.01, ***P < 0.001 vs siRNA-NC group (Unpaired T-test).

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