Fig. 2: LPS induces cardiomyocyte pyroptosis by activating NLRP3 inflammasome and Caspase-1.

A NRCMs were stained with Annexin V/PI and subjected to flow cytometry to detect apoptosis in NRCMs (n = 4). B Western blotting images and the summarized data showing the effect of MCC950 (10 μM) on the protein expression of NLRP3, GSDMD-NT, Caspase-1 p10, IL-18 and IL-1β (n = 4) in NRCMs-induced by LPS. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01. ns: no significant statistical difference.