Fig. 4: IP3R2-mediated Ca2+ release is involved in LPS-induced pyroptosis of cardiomyocytes. | Cell Death Discovery

Fig. 4: IP3R2-mediated Ca2+ release is involved in LPS-induced pyroptosis of cardiomyocytes.

From: IP3R2-mediated Ca2+ release promotes LPS-induced cardiomyocyte pyroptosis via the activation of NLRP3/Caspase-1/GSDMD pathway

Fig. 4

A NRCMs were pre-treated with IP3R inhibitor XeC (0.1 μM) and monitored for changes in intracellular Ca2+ levels stimulated by ATP (10 μM). B Detection of IP3R2 mRNA levels in NRCMs with si-IP3R2 intervention using RT-qPCR analysis (n = 4). C Western blotting images and the summarized data showing the IP3R2 protein expression level significantly decreased in NRCMs with si-IP3R2 intervention (n = 4). D Representative traces of Fluo4/AM changes over time by adding of 10 μM ATP (left). Scatter plot showing the ATP-evoked calcium peak amplitude after adding ATP (right) (n = 20 cells). E, F Flow cytometry analysis was conducted to examine the effects of XeC or si-IP3R2 on apoptosis in NRCMs induced by LPS (n = 4). G, H IL-18 and IL-1β mRNA levels were detected in NRCMs with XeC or si-IP3R2 intervention using RT-qPCR analysis (n = 4). I Western blotting images and summarized data showing the protein expression level of NLRP3, GSDMD-NT, Caspase-1 p10, IL-18 and IL-1β (n = 4) significantly decreased in NRCMs with si-IP3R2 intervention. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01.

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