Fig. 6: The mutual promotion relationships between IP3R2 and ER stress aggravates LPS induced pyroptosis of cardiomyocytes. | Cell Death Discovery

Fig. 6: The mutual promotion relationships between IP3R2 and ER stress aggravates LPS induced pyroptosis of cardiomyocytes.

From: IP3R2-mediated Ca2+ release promotes LPS-induced cardiomyocyte pyroptosis via the activation of NLRP3/Caspase-1/GSDMD pathway

Fig. 6

A Western blotting images and the summarized data showing IP3R2 protein expression level significantly decreased in NRCMs following 4-PBA intervention (n = 4). B Representative traces of Fluo-4/AM changes over time after adding 10 μM ATP (left). Scatter plot showing the ATP-evoked calcium peak amplitude after adding ATP (right) (n = 20 cells). C, D Detection of ATF4 and CHOP mRNA levels in NRCMs with XeC or si-IP3R2 intervention via RT-qPCR analysis (n = 4). E Western blotting images and the summarized data showing the protein expression level of p-eIF2α, CHOP and ATF4 (n = 4) significantly decreased in NRCMs following si-IP3R2 intervention. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01. ns: no significant statistical difference.

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