Fig. 1: Microbial transglutaminase mediated site-specific conjugation of antibody drug conjugates. | Cell Death Discovery

Fig. 1: Microbial transglutaminase mediated site-specific conjugation of antibody drug conjugates.

From: Antibody and antibody fragments site-specific conjugation using new Q-tag substrate of bacterial transglutaminase

Fig. 1

A Schematic representation of antibody with engineered glutamine tag (Q-Tag) and microbial transglutaminase mediated conjugation. B SDS-PAGE analysis of AlexaFluor488-cadaverine or MMAE conjugated engineered Trastuzumab-Qtag antibodies: 1-LLQG, 2-Tag2, 3-Tag3, 4-Tag4, 5-Tag5. ID 6: Trastuzumab-Qtag2-MMAE; ID 7: TrastuzumabK453del-Qtag2-MMAE. The reaction products were separated by 10% SDS-PAGE, then visualized by fluorescent detection and silver-nitrate staining. LC light chain, HC heavy chain, HC dimer heavy chain dimer.

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