Fig. 3: Immunofluorescent staining (left panel) and fluorescent activated cell sorting (FACS) analysis (right panel) on SKBR3 (HER2/neu positive) and MCF7 (HER2/neu status normal) cells. | Cell Death Discovery

Fig. 3: Immunofluorescent staining (left panel) and fluorescent activated cell sorting (FACS) analysis (right panel) on SKBR3 (HER2/neu positive) and MCF7 (HER2/neu status normal) cells.

From: Antibody and antibody fragments site-specific conjugation using new Q-tag substrate of bacterial transglutaminase

Fig. 3

Trastuzumab-Qtag2, Fab-Qtag2, scFv-Qtag2 and VHH-Qtag2 were conjugated with AlexaFluor488-cadaverine and the antigen binding profile was compared to the commercially available Neu-antibody(24D2)-PE. Green, AlexaFluor488 or PE signal; blue, DAPI (nucleus).

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