Fig. 4: Immunofluorescent staining of breast cancer cell lines: SKBR3 (HER2/neu positive). | Cell Death Discovery

Fig. 4: Immunofluorescent staining of breast cancer cell lines: SKBR3 (HER2/neu positive).

From: Antibody and antibody fragments site-specific conjugation using new Q-tag substrate of bacterial transglutaminase

Fig. 4

Evaluation of intracellular internalization of AlexaFluor674 conjugated Trastuzumab-Qtag2 (left panel) or scfv-Qtag2 (right panel) at 4 °C or 37 °C using fluorescent microscopy. Red, AlexaFluor674 signal; green, lysotracker; blue, DAPI (nucleus). AlexaFluor 488 scfv (right panel) at 4 °C or 37 °C using fluorescent microscopy. Green, AlexaFluor 488 – scfv; blue, DAPI.

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